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Humana Cytochrome P450 Protocols: 320 - Methods in Molecular Biology

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Description

The second edition of Cytochrome P450 provides a comprehensive collection of essential core techniques for investigating P450s. This volume from the Methods in Molecular Biology series, number 320, presents the latest methodologies to support advanced research in pharmacology and molecular biology. Researchers will find detailed accounts of protocols for spectral analysis and the purification of P450s. The text covers enzymatic assays for both P450s and flavin-containing monooxygenases (FMOs), as well as methods for the expression of these enzymes in heterologous systems. It also includes guidance on the production and use of antipeptide antibodies. Beyond enzyme studies, this book offers readily reproducible techniques for the transfection of hepatocytes, which is useful for gene regulation studies. This updated edition serves as a practical resource for laboratory professionals seeking reliable, peer-reviewed methods for studying cytochrome P450 and related monooxygenases.

Key Features

Provides updated protocols for the spectral analysis and purification of P450s to support accurate laboratory investigations.

Includes detailed enzymatic assays for both P450s and flavin-containing monooxygenases (FMOs).

Covers methods for the expression of P450s and FMOs within heterologous systems for diverse research applications.

Offers guidance on the production and practical use of antipeptide antibodies in molecular studies.

Contains reproducible techniques for the transfection of hepatocytes specifically for gene regulation studies.

Product Specifications

Brand
Humana
Format
paperback
Domain
Amazon UK
Release Date
19 November 2010
Listed Since
11 July 2010

Barcode

No barcode data available

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