Humana Cytochrome P450: In Vitro Methods and Protocols Book

This specialized volume from Humana provides a comprehensive collection of experimental protocols for in vitro cytochrome P450 (CYP) assays. These methods are essential for researchers and professionals working to select drug candidates within a drug discovery pipeline. The text covers the major factors that influence drug metabolism, providing technical guidance on CYP expression levels and kinetic parameters for individual enzymes. It also addresses critical aspects such as CYP inhibition, induction, and time-dependent inhibition (TDI). Beyond CYP-specific studies, the book explores CYP stability, non-CYP stability, and UDP-glucuronosyltransferases (UGT) stability. Readers will find detailed information regarding excretion mechanisms and drug-drug interactions (DDI). This resource serves as a practical guide for those needing to navigate the complex requirements of pharmacology and toxicology research.