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Elsevier Enzymes in RNA Science and Biotechnology Volume 691

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Description

Enzymes in RNA Science and Biotechnology, Volume 691 in the Methods in Enzymology series, provides comprehensive coverage of cutting-edge advances in enzymatic research and RNA applications. This volume features detailed chapters on reverse transcriptase discovery, preparation, and practical utilisation, including MarathonRT applications for routine RT-PCR and challenging RNA template synthesis. The book explores structured RNAs, repeat RNAs, and engineering approaches such as TNA polymerase development through directed evolution. Additional chapters address RNA structure mapping and determination using reverse transcriptase, along with advanced detection methods including RNA G-quadruplex structure identification via RTS and SHALiPE assays, and tRNA Structure-seq techniques. Designed for researchers, students, and biotechnology professionals, this Academic Press volume serves as an essential reference for understanding enzyme mechanisms in molecular biology and developing practical applications in RNA science.

Key Features

Volume 691 in the Methods in Enzymology series offers authoritative guidance on reverse transcriptase discovery, preparation, and general utilisation techniques for RNA research.

MarathonRT protocols included for routine RT-PCR procedures and cDNA synthesis from structurally complex and challenging RNA templates.

Engineering TNA polymerases through iterative cycles of directed evolution, providing modern approaches to polymerase development and optimisation.

Advanced RNA structure mapping and determination methods using reverse transcriptase Part II techniques for comprehensive molecular analysis.

RNA G-quadruplex detection protocols using RTS and SHALiPE assays enable identification and characterisation of complex secondary structures.

tRNA Structure-seq in vivo methods integrated throughout the volume for studying transfer RNA structures in living systems.

Product Specifications

Format
hardcover
Domain
Amazon UK
Release Date
01 November 2023
Listed Since
17 February 2023

Barcode

No barcode data available

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