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Elsevier Bacterial Efflux Systems Volume 724 - Methods in Enzymology

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Description

Bacterial Efflux Systems, Volume 724 from Methods in Enzymology provides comprehensive coverage of experimental and computational approaches to studying bacterial efflux pump systems. This volume addresses the function, structure, and mechanism of efflux pumps through detailed methodologies including sample preparation for structural and functional analyses, in vivo activity analysis, and efflux pump functional assays. Chapters explore NMR and functional analysis of efflux pumps, saturation mutagenesis techniques, functional characterisation methods, cooperation between pump systems, interaction mapping, HDX-MS approaches, and recombinant protein preparation. Ideal for researchers and biochemists working with bacterial resistance mechanisms, this Methods in Enzymology volume combines practical laboratory protocols with theoretical insights into efflux pump biology and drug resistance pathways.

Key Features

Bacterial Efflux Systems Volume 724 covers both experimental and computational methods for studying efflux pump function, structure, and biochemical mechanisms.

Includes detailed protocols for sample preparation, structural analysis, and functional characterisation of efflux pump systems.

Features chapters on NMR analysis, saturation mutagenesis, interaction mapping, and HDX-MS techniques for efflux pump research.

Addresses cooperation and functional interplay between multiple bacterial efflux pumps in resistance systems.

Provides in vivo activity analysis methods for assessing efflux pump performance in bacterial cells.

Part of the Methods in Enzymology series, offering peer-reviewed protocols from leading researchers in bacterial biochemistry.

Product Specifications

Format
hardcover
Domain
Amazon UK
Release Date
28 November 2025
Listed Since
16 April 2025

Barcode

No barcode data available

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