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UNIV EUROPEENNE Phytoremédiation: Apport de la sélection in vitro pour l'obtention de plantes de colza tolérantes aux métaux toxiques

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Description

La phytoremediation est l'utilisation de plantes pour dépolluer les sols contaminés. Cette technique peut faire l'objet de plusieurs stratégies dont la phytoextraction, qui exploite des plantes capables de concentrer les polluants dans leurs parties aériennes. Néanmoins son efficacité reste limité par la capacité d'extraction et par la biomasse des plantes. L'objectif de ce travail est de sélectionner des variétés de colza tolérantes aux métaux toxiques et si possible hyperaccumulatrices. Une première partie de ce travail, présente la mise au point d'un protocole de néoformation de plantes à partir des CCMts. Une pression de sélection exercée par des métaux (Zn et Pb) additionnées au milieu de culture a permis de régénérer des plantes tolérantes à ces polluants. La deuxième partie aborde les analyses physiologiques et biochimiques de ces régénérants. Les plantes néoformées en présence teneurs en Zinc (100 à 1000 µM) ont été clonées, sevrées, traitées avec 2000 µM de ZnSO4 et analysés. Les teneurs en métabolites majoritaires ont été évaluées par RMN puis les acides aminés libres, la proline, les sucres et les polyamines, les chlorophylles et les caroténoïdes ont été quantifiés.

Product Specifications

Format
paperback
Domain
Amazon UK
Release Date
24 August 2017
Listed Since
18 September 2017

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