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LAP Lambert Academic Publishing Fourier Transform Infrared (FT-IR) Spectroscopy: Fourier Transform Infrared (FT-IR) Spectroscopy, A Novel Technique to Study the Biochemical structure of Bacterial Cells

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Description

This research work was performed to examine the feasibility of using Fourier transform infrared (FT-IR) spectroscopy (in the mid-IR range, 4000-400 cm-1) to i) detect and identify bacterial cells. The greatest spectral variation was observed between 1800 cm-1 and 900 cm-1: for amide I band at ~1650 cm-1, amide II band at ~1540 cm-1, CH3 and CH2 of protein components around 1455 cm-1 and 1398 cm-1, P=O of the phosphodiester backbone of nucleic acids around 1242 cm-1 and 1080 cm-1, and polysaccharide compounds around 1050 - 950 cm-1. From the second derivative transformation and loadings plot, FT-IR showed the potential to detect sublethally injured and dead cells and discriminate between different types and degrees of cell injury.

Product Specifications

Format
paperback
Domain
Amazon UK
Release Date
07 February 2011
Listed Since
10 February 2011

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