Humana Glycosylation Engineering of Biopharmaceuticals: Methods and Protocols: 988 (Methods in Molecular Biology, 988)

This Methods in Molecular Biology™ book offers protocols for glycoproteins and glyco-engineered biopharmaceuticals with a focus on mAbs. Covers glyco-engineering of therapeutic proteins, glycoanalytics, glycoprotein complex characterization and PK/PD assays. Review From the reviews: “The book is intended as a reference manual of protocols and best practices for the practicing bench scientist. The authors do a marvelous job of covering a wide variety of tools and techniques and bringing them together in one volume. … This collection of protocols will find an audience with experimentalists in the biopharmaceutical industry as well as various academic labs. … Overall, I would strongly recommend this book … .” (Bibhash Mukhopadhyay, Frontiers in Pharmacology, September, 2013)“This book … useful as it details in one place the current state of art technologies and techniques for antibodies. I enjoyed reading the introductions to each chapter, which were accessible and gave an historical setting and overall aim for the methodologies that are set out in the chapter. … this book offers a good reference guide for those of us working in this area.” (Sian Estdale, BTS Newsletter – The British Toxicology Society, Issue 43, Winter, 2013) From the Back Cover Glyco-engineering is being developed as a method to control the composition of carbohydrates and to enhance the pharmacological properties of monoclonal antibodies (mAbs) and other proteins.  In Glycosylation Engineering of Biopharmaceuticals: Methods and Protocols, experts in the field provide readers with production and characterization protocols of glycoproteins and glyco-engineered biopharmaceuticals with a focus on mAbs. The volume is divided in four complementary parts dealing with glyco-engineering of therapeutic proteins, glycoanalytics, glycoprotein complexes characterization, and PK/PD assays for therapeutic antibodies.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and cutting-edge, Glycosylation Engineering of Biopharmaceuticals: Methods and Protocols serves as an ideal guide for scientists striving to push forward the exciting field of engineered biopharmaceuticals.