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Humana Recombinant Protein Production in Yeast - Methods & Protocols

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Description

This comprehensive guide from Humana examines advanced techniques for producing recombinant proteins in yeast systems, covering critical aspects of host strain engineering and expression optimisation. The book explores how yeast cells respond to recombinant protein production stress and details methods for leveraging this knowledge to develop improved host strains. Key topics include expression vector design, selection of promoters and tags, and codon optimisation strategies for target genes. The work addresses preparation of expression vectors, transformation procedures for generating high-yielding clones, and optimisation protocols essential for successful recombinant protein experiments. Ideal for molecular biologists, biotechnologists, and researchers working with protein expression systems, this Methods in Molecular Biology volume provides practical protocols and detailed methodology for maximising protein yields and improving production efficiency in yeast-based systems.

Key Features

Recombinant Protein Production in Yeast covers expression vector design, promoter selection, and codon optimisation strategies for targeted protein genes.

The book includes protocols for yeast cell transformation and methods for generating high-yielding recombinant protein clones.

Part of the Methods in Molecular Biology series (volume 866), offering peer-reviewed laboratory techniques and experimental procedures.

Addresses host strain engineering approaches and yeast cellular stress responses to recombinant protein production.

Humana's Methods in Molecular Biology resource provides practical guidance on expression vector preparation and protein production optimisation in yeast systems.

Product Specifications

Brand
Humana
Format
hardcover
Domain
Amazon UK
Release Date
28 March 2012
Listed Since
24 November 2011

Barcode

No barcode data available

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