Elsevier Academic Press DNA Repair Enzymes Volume 592 - Methods in Enzymology

DNA Repair Enzymes: Structure, Biophysics, and Mechanism (Volume 592) is a specialized addition to the Methods in Enzymology series by Academic Press. As the first part of a thematic series focused on DNA repair, this volume provides deep technical insights into the molecular processes of genetic maintenance. Readers can explore advanced topics such as the production and assay of recombinant multisubunit chromatin remodeling complexes and new technologies like MacroBac for the efficient large-scale production of recombinant multiprotein complexes. The text also covers the analysis of functional dynamics in modular multidomain proteins using SAXS and NMR. Additionally, it examines the use of single-cysteine variants for trapping transient states in DNA mismatch repair and provides structural studies of RNase. This volume serves as a technical resource for researchers and medical healthcare practitioners working in internal medicine and molecular biology.