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Elsevier Apobec Enzymes Volume 713 - Methods in Enzymology

Amazon

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Description

Apobec Enzymes, Volume 713 in the Methods in Enzymology series, presents cutting-edge research on APOBEC protein function and characterisation. This comprehensive reference compiles contributions from an international team of experts, covering advanced experimental techniques for studying RNA editing mechanisms. The volume includes detailed chapters on fluorescent shift assays for APOBECs RNA editing, low error sequencing methods to detect APOBEC-mediated RNA editing through Circular RNAseq and Safe-Sequencing System approaches, and the Safe-Barcode RNAseq assay for APOBECs RNA editing detection. Additional chapters explore the DT40 cell system for characterising somatic hypermutation and the CH12 cell system for assaying AID activity on class switch recombination. The book also covers purification of enzymatically active APOBEC proteins, providing essential protocols for researchers working in molecular biology, biochemistry, and enzyme research.

Key Features

Volume 713 in the established Methods in Enzymology series from Academic Press, offering peer-reviewed experimental protocols for APOBEC enzyme research.

Features fluorescent shift assay techniques specifically designed for studying APOBECs RNA editing activity and mechanisms.

Includes low error sequencing methods and the Safe-Sequencing System for accurately detecting APOBEC-mediated RNA editing in molecular biology research.

Provides the Safe-Barcode RNAseq assay protocol for comprehensive analysis of APOBECs RNA editing patterns.

Covers DT40 and CH12 cell system methodologies for characterising somatic hypermutation and assaying AID activity on class switch recombination.

Contains protocols for purification of enzymatically active APOBEC proteins essential for biochemistry and molecular research applications.

Product Specifications

Format
hardcover
Domain
Amazon UK
Release Date
01 April 2025
Listed Since
20 November 2024

Barcode

No barcode data available

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