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Academic Press Apobec Enzymes: Volume 713 - Methods in Enzymology

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Description

Expand your research knowledge with Apobec Enzymes: Volume 713 from the Methods in Enzymology series. Published by Academic Press, this volume presents recent advances in the field through chapters written by an international board of authors. This academic resource provides deep insights into the technical aspects of APOBEC-mediated RNA editing and enzyme activity. Researchers can explore specialized methodologies such as the Fluorescent shift assay for APOBECs RNA editing and low error sequencing methods like Circular RNAseq and the Safe-Sequencing System. The text also covers the "Safe-Barcode" RNAseq assay and utilizes the DT40 cell system to characterize somatic hypermutation. Additionally, it details the CH12 cell system for assaying AID activity on class switch recombination and provides guidance on the purification of enzymatically active APOBEC. This volume serves as a vital technical reference for scientists working in biochemistry and molecular biology.

Key Features

Includes advanced research on Fluorescent shift assay for APOBECs RNA editing to support laboratory investigations.

Covers Low Error Sequencing Methods including Circular RNAseq and Safe-Sequencing System for precise detection of RNA editing.

Provides technical details on the "Safe-Barcode" RNAseq assay designed specifically for APOBECs RNA editing studies.

Explores the DT40 cell system for the characterization of somatic hypermutation in biological research.

Details the use of the CH12 cell system to assay AID activity on class switch recombination.

Offers methodologies for the purification of enzymatically active APOBEC to assist in biochemical studies.

Product Specifications

Format
hardcover
Domain
Amazon UK
Release Date
01 April 2025
Listed Since
20 November 2024

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