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Aspekte differenzierter Stammzellen für die Rekonstitution in vivo

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£42.00 £39.90 £40.74 £41.58 £42.42 £43.26 £44.10 27 July 2026 08 August 2026 21 August 2026 03 September 2026 16 September 2026
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Description

Unser Ziel war es, ein In-vitro-Differenzierungssystem zu etablieren, um embryonale Stammzellen (ES-Zellen) der Linie BALB/c IgM-/- für die Lymphozytenentwicklung vorzubereiten. Wir haben die BALB/c IgM-/- ESC-Linie mittels Fluoreszenz-aktivierter Zellsortierung (FACS) und Epifluoreszenz auf Marker der Pluripotenz untersucht. So haben wir diese Zelllinie hinsichtlich der Expression der kongenischen Marker CD45.1 und CD45.2 auf ihrer Oberfläche charakterisiert. Da die B-Zell-Entwicklung im Knochenmark stattfindet, sollten die differenzierten embryonalen Stammzellen die Fähigkeit besitzen, nach dem Transfer in die Entwicklungsnischen zu wandern. Daher untersuchten wir die Homing-Fähigkeit mittels Transwell-Migrationsassays gegen das C-X-C-Motiv-Chemokin 12 (CXCL12 = SDF1), das einen starken Lockstoff für Stammzellen und Vorläuferzellen darstellt und die Zellen zu ihren Entwicklungsnischen im Knochenmark leitet.

Product Specifications

Format
paperback
Domain
Amazon UK
Release Date
27 April 2026
Listed Since
29 April 2026

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