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Détection quantitative d'E.coli O157:H7 dans la viande de bœuf hachée

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Description

Escherichia coli O157:H7 est reconnu comme un entéropathogène humain majeur. Une immuno-agglutination spectrophotométrique directe - le premier test jamais publié - a été développée pour la quantification des IgG spécifiques d'Escherichia coli O157. Une nouvelle solution de lyse cellulaire appelée TZ a également été mise au point et s'est avérée supérieure à diverses méthodes de lyse cellulaire avant la PCR. Le développement d'une procédure d'extraction différentielle a permis de multiplier par 6 le nombre de cellules d'E. coli 0157:H7 extraites à partir de viande de bœuf hachée ensemencée. L'amplification la plus élevée d'E. coli O157 : H7 a été obtenue à l'aide de la solution de lyse TZ, des procédures d'extraction et de l'optimisation de la procédure PCR ; elle a permis de détecter 100 cellules de SLT-1 et 50 cellules de SLT-2 par 10 grammes de viande hachée.

Product Specifications

Format
paperback
Domain
Amazon UK
Release Date
16 September 2021
Listed Since
17 September 2021

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