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EAE Editorial Academia Espanola Estudio de apoptosis en embriones bovinos: Influencia de los factores de crecimiento: EGF, FGF, IGF-I y PDGF en la producción in vitro de embriones

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Description

El objetivo de este trabajo fue estudiar el efecto de la adición de factores de crecimiento a los medios utilizados en la producción in vitro de embriones de ganado bovino. Los factores de crecimiento EGF, FGFb, IGF-I, PDGF, y la mezcla de todos ellos, EFIP, fueron añadidos al medio de maduración, al de cultivo o bien a ambos, comparándolos con la adición de FCS o con un medio carente de fuente proteica. Los resultados obtenidos tras la adición de FCS fueron los mejores al proporcionar los mayores porcentajes de eclosión. Por otra parte, la utilización de FCS resultó ser más beneficiosa cuando se hizo a partir del día 3 postinseminación. Se demostró que el cultivo en grupos pequeños fue desfavorable para la producción in vitro de embriones bovinos y que el desarrollo embrionario temprano mejoraba cuando se cultivaba en microgota, frente al cultivo en pocillo. En este trabajo se valoró la calidad de los blastocistos expandidos por microscopia confocal, analizando el número de células y los índices de mitosis, apoptosis, apoptosis temprana y necrosis, en la MCI, en el TE y en el blastocisto total. El grupo cultivado con FCS proporcionó embriones con los mejores índices de calidad.

Product Specifications

Format
paperback
Domain
Amazon UK
Release Date
06 April 2012
Listed Since
13 July 2012

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