Cloning & Isolation of Taq DNA polymerase using pET expression system: Molecular Cloning - Image 1

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LAP Lambert Academic Publishing Cloning & Isolation of Taq DNA polymerase using pET expression system: Molecular Cloning

Name: Cloning & Isolation of Taq DNA polymerase using pET expression system: Molecular Cloning
Brand: LAP Lambert Academic Publishing
Price: 43.0 GBP
Availability: InStock

Biological Science

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Description

The thermostable properties of the Taq DNA Polymerase from Thermus aquaticus have contributed greatly to yield, specificity, automation and utility in the PCR for amplification of DNA. Isolation of this protein from T. aquaticus is difficult. Several studies have shown the recombinant expression of Taq DNA Polymerase in heterologous host cells. However, these still suffer from low yields. The use of T7 RNA Polymerase regulated expression vector and availability of affinity tag (His Tag) can make the expression of Taq DNA Polymerase an efficient and economical process. The project is aimed in recombinant expression of Taq DNA Polymerase in BL21 host cell line and pET expression vector.

Product Specifications

Brand: LAP Lambert Academic Publishing
Format: paperback
ASIN: 3845443707
Category: Books > Subjects > Science, Nature & Maths > Biological Sciences > Biological Science
Domain: Amazon UK
Release Date: 20 September 2011
Listed Since: 21 September 2011

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LAP Lambert Academic Publishing Cloning & Isolation of Taq DNA polymerase using pET expression system: Molecular Cloning

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