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LAP Lambert Academic Publishing TOXIGENIC BACTERIAL PROTEASES AND THEIR INHIBITORS: Bacterial proteases production, purification and partial characterization

Name: TOXIGENIC BACTERIAL PROTEASES AND THEIR INHIBITORS: Bacterial proteases production, purification and partial characterization
Brand: LAP Lambert Academic Publishing
Price: 57.0 GBP
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Microbiology

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Description

Among 128 proteolytic bacterial isolates, Brevibacterium otitidis, CBS-76 was choosen on the basis of secreted protease. Analysis of purified protease revealed an apparent molecular weight of 47 KDa with 15.2% glutamic acid content. The purified protease showed the maximum activity after 68h at 35ºC and pH 7.2. The purified enzyme was inhibited in the presence of EDTA but maximally activated in the presence of sodium arsenate. LD50 on mice was 8 ?g /100 g body weight. Histological study using transmission electron microscope revealed that nuclei of treated mice hepatocytes appeared with inactive heterochromatin and the nucleoli tend to disintegrate. While the cytoplasm contains swollen mitochondria and some destructed mitochondria lost their inner cristae. And fat droplets accumulated in the cytoplasm in the form of empty vacuoles(steatosis). On studying, novel inhibitors derived from common drugs derivatives of saccharin sodium,compound 6 and 9 showed the maximum percentage of enzyme inhibition.

Product Specifications

Brand: LAP Lambert Academic Publishing
Format: paperback
ASIN: 3843391769
Category: Books > Subjects > Science, Nature & Maths > Biological Sciences > Microbiology
Domain: Amazon UK
Release Date: 14 January 2011
Listed Since: 21 January 2011

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LAP Lambert Academic Publishing TOXIGENIC BACTERIAL PROTEASES AND THEIR INHIBITORS: Bacterial proteases production, purification and partial characterization

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LAP Lambert Academic Publishing TOXIGENIC BACTERIAL PROTEASES AND THEIR INHIBITORS: Bacterial proteases production, purification and partial characterization

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