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VDM Verlag Protocolo biológico de reconocimiento del TNFα mediante SPR: Detección del Factor de Necrosis Tumoral α (TNFα) mediante técnicas de resonancia plasmónica superficial (SPR)

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Description

El TNFα, citoquina proinflamatoria con diversas funciones en el organismo, está implicado en múltiples acciones fisiológicas como la hematopoyesis, el sistema inmune y la regresión tumoral. Es la molécula diana de muchos tratamientos efectivos para enfermedades inflamatorias como el síndrome de Crohn y la artritis reumatoide. Sin embargo, a día de hoy no se determina de manera rutinaria para el diagnóstico en análisis clínico. Ni siquiera en pacientes tratados con terapias anti TNFα. Las técnicas actuales para medir su concentración (ELISA) sólo se utilizan en estudios de investigación por su elevado coste. Este trabajo ha pretendido impulsar el conocimiento sobre el TNFα mediante una revisión exhaustiva de artículos y libros científicos, y la caracterización de su detección diseñando un protocolo para la formación de una estructura de reconocimiento biológico con el uso de las SAMs mediante técnicas de SPR. Por último, utilizando la estructura de biorreconocimiento de TNFα óptima, se han analizado muestras de plasma de ratas sanas y con esteatosis inducida, pudiendo distinguir entre las que tenían concentraciones basales y elevadas de TNFα en plasma con el sistema diseñado.

Product Specifications

Format
paperback
Domain
Amazon UK
Publication Date
21 July 2014
Listed Since
14 August 2014

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