Humana Extracellular Matrix Protocols: Second Edition: 522 (Methods in Molecular Biology, 522)

Expert researchers explore this developing field and provide techniques which can be reproduced in most cell biology labs. This new edition contains cutting-edge methods that are worth publicizing in the field and may encourage collaboration with expert labs. Review From the reviews of the second edition: “Significant advances in the field of extracellular matrix during the past decade and current extensive investigations in this field have necessitated the second edition of this book on extracellular matrix protocols. … well illustrated with of figures, photographs, and graphs in color and in black-and-white. … students interested in cell biology will find this book quite useful. … this second edition is surely welcome in the research community. Both expert and nonexpert researchers in the field of extracellular matrix will find this book extremely useful.” (Omer Iqbal, Doody’s Review Service, July, 2009) From the Back Cover The study of the extracellular matrix has significantly expanded over the past decade. Although thoroughly researched, the complexity and the dynamic nature of the matrix are still under extensive investigation, yielding a growing number of methods of analysis. In Extracellular Matrix Protocols: Second Edition, expert researchers explore this developing field and provide a number of techniques which can be reproduced in most cell biology labs. This edition contains methods that represent cutting edge unique technologies which are well worth exposure to the broad field and may encourage collaboration with expert labs. Chapters include information on molecular biology techniques to study gene expression, biophysical and biochemical methods for the analysis of structure and composition, and cell biology methods for assessment of cell behavior, matrix assembly and tissue engineering applications. Composed in the highly successful Methods in Molecular Biology(tm) series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Extracellular Matrix Protocols: Second Edition investigates the diverse roles of the extracellular matrix, providing important tools for all researchers in the ECM community.