Humana Telomeres and Telomerase: Methods and Protocols: 735 (Methods in Molecular Biology, 735)

New and rapid advances in technology have equipped us with a variety of tools and platforms to ask fundamental questions of telomere regulation and have allowed investigators to carry out experiments using diverse model systems.  For example, proteomic, genomic, and molecular approaches have afforded us unprecedented insight into the complex protein interaction networks at work on the telomere chromatin and the detailed information regarding telomere dynamics in response to stress or stimuli.  Telomeres and Telomerase: Methods and Protocols, Second Edition builds upon the telomerase assays featured in the popular first edition to encompass many different assays that allow investigators to query the function of telomere proteins and the responses of the telomere DNA, including detailed examinations of biochemical, molecular, and proteomic approaches.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Telomeres and Telomerase: Methods and Protocols, Second Edition serves as an ideal, up-to-date guide for investigators further pursing this vital field of study. Review From the reviews of the second edition:“The book provides different assays to study the function of telomere proteins and the responses of the telomere DNA. It also provides protocols for biochemical, molecular, and proteomic approaches in the understanding of telomere dynamics. It appears to be intended for investigators interested in the field of telomere and telomerase, but it also will be of immense help to other clinicians interested in cell growth, proliferation, aging, and cancer.” (Omer Iqbal, Doody’s Book Reviews, February, 2012) From the Back Cover New and rapid advances in technology have equipped us with a variety of tools and platforms to ask fundamental questions of telomere regulation and have allowed investigators to carry out experiments using diverse model systems.  For example, proteomic, genomic, and molecular approaches have afforded us unprecedented insight into the complex protein interaction networks at work on the telomere chromatin and the detailed information regarding telomere dynamics in response to stress or stimuli.  Telomeres and Telomerase: Methods and Protocols, Second Edition builds upon the telomerase assays featured in the popular first edition to encompass many different assays that allow investigators to query the function of telomere proteins and the responses of the telomere DNA, including detailed examinations of biochemical, molecular, and proteomic approaches.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Telomeres and Telomerase: Methods and Protocols, Second Edition serves as an ideal, up-to-date guide for investigators further pursing this vital field of study.