Nuclear Reprogramming: Methods and Protocols: 325 (Methods in Molecular Biology, 325) - Image 1

£74.26

Humana Nuclear Reprogramming: Methods and Protocols: 325 (Methods in Molecular Biology, 325)

Name: Nuclear Reprogramming: Methods and Protocols: 325 (Methods in Molecular Biology, 325)
Brand: Humana
Price: 74.26 GBP
Availability: InStock

Biochemistry

Price History & Forecast

Last 8 days • 8 data points (No recent data available)

Historical

Generating forecast...

Price Distribution

Price distribution over 8 days • 1 price levels

Days at Price

Price Analysis

Most common price: £74 (8 days, 100.0%)

Price range: £74 - £74

Price levels: 1 different prices over 8 days

Description

Product Description A wide-ranging collection of readily reproducible methods for performing nuclear reprogramming by nuclear transfer in several different species, by fusion through both chemical treatment and electrically shocking cells, and by in vivo treatment of cells with cell extracts. Several methods of monitoring nuclear reprogramming are also presented, including the use of transgenic markers, activation of telomerase as an ES-specific marker, light and electron microscopic observation of structural changes in the nucleus, and verification of surface marker expression and the differentiation potential of stem cells. Biochemical methods are provided for the examination of chromatin protein modifications, nucleosomal footprinting, transcription factor binding, and the study of DNA methylation changes both at the specific locus level and at the level of the whole nucleus. From the Back Cover Although nuclear reprogramming-the cellular process underlying cloning and the artificial generation of stem cells from somatic cells-may be considered a "black box" whose interior events are either completely unknown or at least imperfectly characterized, tantalizing clues about its operation are beginning to emerge. In Nuclear Reprogramming: Methods and Protocols, hands-on laboratory scientists describe readily reproducible methods for actually performing nuclear reprogramming by nuclear transfer in several different species, by fusion induced both chemically and electrically, and by the in vivo treatment of cells with cell extracts. Isolation of an adult stem cell type is also described. In addition, several methods of monitoring nuclear reprogramming are presented, including the use of transgenic markers, activation of telomerase as an ES-specific marker, light and electron microscopic observation of structural changes in the nucleus, and the verification of surface marker expression and differentiation potential of stem cells. Biochemical methods are provided for the examination of chromatin protein modifications, nucleosomal footprinting, transcription factor binding, and the study of DNA methylation changes both at the specific locus level and at the level of the whole nucleus. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Wide-ranging and highly practical, Nuclear Reprogramming: Methods and Protocols significantly expands the repertoire of methods available to perform, study, and understand nuclear programming, not only for cloning, but also for reprogramming somatic cells to stem cells.

Product Specifications

Brand: Humana
Format: Paperback
ASIN: 1617375306
Category: Books > Subjects > Science, Nature & Maths > Biological Sciences > Biochemistry
Domain: Amazon UK
Release Date: 20 December 2010
Listed Since: 01 October 2010

Barcode

No barcode data available

Humana Nuclear Reprogramming: Methods and Protocols: 325 (Methods in Molecular Biology, 325)

Price History & Forecast

Price Distribution

Price Analysis

Description

Product Specifications

Barcode

Similar Products You Might Like

Nuclear Reprogramming: Methods and Protocols: 1222 (Methods in Molecular Biology, 1222)

Cell Reprogramming: Methods and Protocols: 1330 (Methods in Molecular Biology, 1330)

Cell Reprogramming: Methods and Protocols: 1330 (Methods in Molecular Biology, 1330)

Nuclear Reprogramming and Stem Cells (Stem Cell Biology and Regenerative Medicine)

Neural Reprogramming: Methods and Protocols: 2352 (Methods in Molecular Biology, 2352)

Cellular Programming and Reprogramming: Methods and Protocols: 636 (Methods in Molecular Biology, 636)

Mammalian Chromosome Engineering: Methods and Protocols: 738 (Methods in Molecular Biology)

DNA Repair Protocols: 113 (Methods in Molecular Biology, 113)

Pluripotent Stem Cells: Methods and Protocols: 997 (Methods in Molecular Biology, 997)

Genomic Imprinting: Methods and Protocols: 925 (Methods in Molecular Biology, 925)

Pluripotent Stem Cells: Methods and Protocols: 997 (Methods in Molecular Biology, 997)

Chromatin Remodeling: Methods and Protocols: 833 (Methods in Molecular Biology, 833)

Protocols for Nucleic Acid Analysis by Nonradioactive Probes: 353 (Methods in Molecular Biology, 353)

The Nuclear Receptor Superfamily: Methods and Protocols: 505 (Methods in Molecular Biology, 505)

Mobile Genetic Elements: 260 (Methods in Molecular Biology, 260)

RNA Remodeling Proteins: Methods and Protocols: 1259 (Methods in Molecular Biology, 1259)

Telomeres and Telomerase: Methods and Protocols: 735 (Methods in Molecular Biology, 735)

Human Pluripotent Stem Cells: Methods and Protocols: 767 (Methods in Molecular Biology, 767)

Xenopus Protocols: Post-Genomic Approaches: 917 (Methods in Molecular Biology, 917)

Synthetic Messenger RNA and Cell Metabolism Modulation: Methods and Protocols: 969 (Methods in Molecular Biology, 969)

Humana Chromatin Remodeling: Methods and Protocols: 833

Somatic Stem Cells: Methods and Protocols: 879 (Methods in Molecular Biology, 879)

Humana Stem Cell Transcriptional Networks Methods and Protocols

Humana Human Pluripotent Stem Cells: Methods and Protocols 767