Humana Colorectal Cancer: Methods and Protocols: 50 (Methods in Molecular Medicine, 50)

The contents of Colorectal Cancer: Methods and Protocols aim to instruct investigators in all the key genetic, cellular, and molecular biological methods of analyzing colorectal tumors. The focused techniques and assays are described in sufficient detail to allow researchers to start an experiment on colon tumors and proceed from beginning to end as if the expert in the field who has performed these studies were guiding them at the bench. Of note, most of the chapters in this volume are written by those scientists who p- neered these methods and assays in their respective fields. The chapters in Colorectal Cancer: Methods and Protocols describe "state of the art" methods to analyze colorectal tumors, ranging from gross mic- dissection of specimens to specific molecular analyses. Included are coverages of mutational assays, instability testing, immunohistochemical assays, chro- somal studies, and gene expression analyses. The goal of our volume is to facilitate the performance of colorectal tumor biological experiments by investigators at various levels of training-from graduate students and postdoctoral fellows to principal investigators who desire to advance our understanding of colon cancer development. Review "This is quite interesting and a valuable volume. It aims to instruct investigators in the key genetic, cellular and molecular biological methods for analyzing colorectal tumors. It's quite precise in its description of these techniques and, as the preface says, "The focused techniques and assays are described in sufficient detail to allow researchers to start an experiment on colon tumors and proceed from beginning to end as if the expert in the field who has performed these studies were guiding them at the bench." A large amount of practical information is packed into one small volume and this volume is ideal for students and fellows who wish to expand their knowledge in this area and have a ready volume reference in developing new techniques...The volume is also good value for those of us who don't get into the lab as it clarifies many of the techniques which are used and helps the non-involved reader to understand the terminology and techniques used in this important area of cancer research." - Cancer Forum "...Dr. Saltz has produced as concise a presentation as possible within the framework of the widely scattered disciplines searching the complexity of diagnostic and therapeutic problems occurring among patients with colorectal cancer." - Comprehensive Therapy From the Back Cover Colorectal cancer is a common and highly lethal cancer once spread whose treatment is improving as we gain a better understanding of its development. In Colorectal Cancer: Methods and Protocols, Steven M. Powell, MD, has assembled a hands-on collection of cutting-edge molecular methods for studying this solid tumor. These readily reproducible techniques range from gross microdissection of specimens to specific molecular analyses. Here both the novice and experienced researcher will find productive chromosomal analyses, including comparative genomic hybridization and FISH assays, and revealing gene expression analyses, including immunohistochemical assays, Western blotting, and microarray assays. Also presented are mutational analyses of colon tumors and blood samples to determine whether specific patients have inherited a significant predisposition to colorectal cancer. Each method is described in step-by-step detail by an established expert to ensure robust experimental results, and all are accompanied by detailed notes on pitfalls to avoid. Eminently practical, with each technique presented as if the expert in the field who had perfected it were guiding the investigator, Colorectal Cancer: Methods and Protocols provides cancer investigators with a gold standard collection of all the key genetic, cellular, and molecular biological methods for analyzing colon cancer today.