Humana Chlamydia trachomatis: Methods and Protocols: 2042 (Methods in Molecular Biology, 2042)

This book explores cutting-edge methods to work with the notoriously difficult, but highly prevalent, obligate intracellular pathogen,  Chlamydia trachomatis. These include techniques to identify  Chlamydia trachomatis in patient samples, ranging from simple point-of-care tests to whole genome sequencing; methods for propagation of strains in both cell culture and animal models; techniques to manipulate  Chlamydia trachomatis in molecular genetic methodologies; a high-throughput screening method for testing new potential drugs against intracellular bacteria; a screen for antibiotic resistance; methods for labeling and enumeration; and descriptions of genotyping technologies, as well as dual RNA-Seq transcriptional profiling. Written for the highly successful  Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative, practical, and relevant,  Chlamydia trachomatis: Methods and Protocols serves as an ideal reference for scientists searching for a better understanding of the pathogen, allowing for the development of improved treatment regimens and the discovery of new drugs. Review “Students, postgraduates, scientists and researchers, public health researchers, and microbiologists will find this book of immense interest. … This is an important book with current information on cutting‐edge methods and protocols for Chlamydia trachomatis. Given the rapid advances in this field, this book will appeal to its intended audience.” (Omer Iqbal, Doody’s Book Reviews, January 27, 2020) From the Back Cover This book explores cutting-edge methods to work with the notoriously difficult, but highly prevalent, obligate intracellular pathogen,  Chlamydia trachomatis. These include techniques to identify  Chlamydia trachomatis in patient samples, ranging from simple point-of-care tests to whole genome sequencing; methods for propagation of strains in both cell culture and animal models; techniques to manipulate  Chlamydia trachomatis in molecular genetic methodologies; a high-throughput screening method for testing new potential drugs against intracellular bacteria; a screen for antibiotic resistance; methods for labeling and enumeration; and descriptions of genotyping technologies, as well as dual RNA-Seq transcriptional profiling. Written for the highly successful  Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative, practical, and relevant,  Chlamydia trachomatis: Methods and Protocols serves as an ideal reference for scientists searching for a better understanding of the pathogen, allowing for the development of improved treatment regimens and the discovery of new drugs.