Humana Human Monoclonal Antibodies: Methods and Protocols: 1904 (Methods in Molecular Biology, 1904)

Dedication... Preface... Table of Contents... Contributing Authors... 1. Human Monocolonal Antibodies: The Benefits of Humanization Herman Waldmann 2. Cancer Immunotherapy: The Dawn of Antibody Cocktails Ilaria Marrocco, Donatella Romaniello, and Yosef Yarden 3. IgM Natural Autoantibodies in Physiology and the Treatment of Disease Mahboobeh Fereidan-Esfahani, Tarek Nayfeh, Arthur Warrington, Charles L. Howe, and Moses Rodriguez 4. Monoclonal Antibodies for the Treatment of Melanoma: Present and Future Strategies Madhuri Bhandaru and Anand Rotte 5. An Efficient Method to generate Monoclonal Antibodies from Human B Cells Jenna J. Guthmiller, Haley L. Dugan, Karlynn E. Neu, Linda Yu-Ling Lan, and Patrick C. Wilson 6. Isolation of Antigen-Specific, antibody-Secreting Cells using a Chip-Based Immunospot Array Hiroyuki Kishi, Tatsuhiko Ozawa, Hiroshi Hamana, Eiji Kobayashi, and Atsushi Muraguchi 7. Purification of Human Monoclonal antibodies and Their Fragments Nicole Ulmer, Sebastian Vigg, Thomas Müller-Späth, and Massimo Morbidelli 8. One-Tube Multicolor Flow Cytometry Assay (OTMA) for Comprehensive Immunophenotyping of Peripheral Blood Anna-Jasmina Donaubauer, Paul F. Rühle, Ina Becker, Rainer Fietkau, Udo S. Gaipl, and Benjamin Frey 9. Humanization and Simultaneous Optimization of Monoclonal Antibody T. Kuramochi, T. Igawa, H. Tsunoda, and K. Hattori 10. Antibody Fragments Humanization: Beginning with the End in Mind Nicolas Aubrey and Philippe Billiald 11. Antigen-Specific Human Monoclonal Antibodies from Transgenic Mice Susana Magadán Mompó and África González-Fernández 12. Refining the Quality of Monoclonal Antibodies: Grafting Unique Peptide-Binding  Site in the Fab Framework Jeremy D. King and John C. Williams 13. Basic Procedures for Detection and Cytotoxicity of Chimeric Antigen Receptors Keichiro Mihara, Tetsumi Yoshida, and Joyeeta Bhattacharyya 14. Rapid Chimerization of Antibodies Koji Hashimoto, Kohei Kurosawa, Hidetaka Seo, and Kunihiro Ohta 15. Phage Display Technology for Human Monoclonal Antibodies Dal Ferro Marco, Rizzo Serena, Rizzo Emanuela, Marano Francesca, Luisis Immacoloata, Tarasiuk Olga, and Daniele Sblattero 16. Recombinant Antibody Selections by Combining Phage and Yeast Display Fortunato Ferrara, Maria Felicia Soluri, and Daniele Sblattero 17. Epitope Mapping via Phage Display from Single Gene Libraries Viola Fühner, Philip Alexander Heine, Kilian Zilkens, Doris Meier, Kristian Daniel Ralph Roth, Gustavo Marçal Schmidt Garcia Moreira, Michael Hust, and Giulio Russo 18. A High-Throughput Magnetic Nanoparticle Based Semi-Automated Antibody Phage Display Biopanning Angela Chiew Wen Ch'ng, Azimah Ahmad, Zoltán Konthur, and Theam Soon Lim 19. A Binding Potency Assay for Pritumumab and Ecto-Domain Vimentin  Ivan Babic, Santosh Kesari, and Mark C. Glossy 20. A Method to Detect the Binding of Hyper-Glycosylated Fragment Crystallizable (Fc) Region of Human IgG1 to Glycan Receptors Patricia Blundell and Richard Pleass 21. A Cell-Based Reporter Assay Measuring the Activation of Fc Gamma Receptors Induced by Therapeutic Monoclonal Antibodies Michihiko Aoyama, Minoru Tada, and Akiko Ishii-Watabe 22. "BIClonals": Production of Bispecific Antibodi From the Back Cover This second edition volume expands on the previous edition with descriptions of recent developments in the field. The chapters in this book cover topics such as monoclonal antibodies for the treatment of melanoma; production and purification of human monoclonal antibodies; humanization and optimization of monoclonal antibodies; rapid chimerization of monoclonal antibodies; epitope mapping via phage display from single gene libraries; recombinant antibodies made by combining phage and yeast display selections; production of stabilized antibody fragments in the E. c