Humana Molecular Toxicology Protocols: 1105 (Methods in Molecular Biology, 1105)

Part I. Toxicoproteomics 1. Array-Based Immunoassays with Rolling-Circle Amplification Detection Katie Partyka, ShuangshuangWang, Ping Zhao, Brian Cao, and Brian Haab 2. Analysis of Protein Changes Using Two Dimensional Difference Gel Electrophoresis Weimin Gao Part II. Toxicant-Induced Inflammation 3. Assessment of Pathological and Physiological Changes in Mouse Lung Through Bronchoalveolar Lavage Yuanpu Peter Di 4. Analysis of Clinical and Biological Samples Using Microsphere-Based Multiplexing Luminex System Yingze Zhang, Rahel Birru, and Y. Peter Di Part III. Gene Promoter Methylation 5. Detection of DNA Methylation by MeDIP and MBDCap Assays: An Overview of Techniques Hang-Kai Hsu, Yu-I Weng, Pei-Yin Hsu, Tim H.-M. Huang, and Yi-Wen Huang 6. Screening of DNA Methylation Changes by Methylation-Sensitive Random Amplified Polymorphic DNA-Polymerase Chain Reaction (MS-RAPD-PCR) Kamaleshwar P. Singh Part IV. Array Technologies 7. Strategies for Measurement of Biotransformation Enzyme Gene Expression Marjorie Romkes and Shama C. Buch 8. Genotyping Technologies: Application to Biotransformation Enzyme Genetic Polymorphism Screening Marjorie Romkes and Shama C. Buch 9. TaqMan Fluorogenic Detection System to Analyze Gene Transcription in Autopsy Material Kaori Shintani-Ishida, Bao-Li Zhu, and Hitoshi Maeda Part V. Analysis of DNA Adducts 10. 32P-Postlabelling Analysis of DNA Adducts David H. Phillips and Volker M. Arlt 11. Modification of the 32P-Postlabeling Method to Detect a Single Adduct Species as a Single Spot Masako Ochiai, Takashi Sugimura, and Minako Nagao 12. DNA Isolation and Sample Preparation for Quantitation of Adduct Levels by Accelerator Mass Spectrometry Karen H. Dingley, Esther A. Ubick, John S. Vogel, Ted J. Ognibene, Michael A. Malfatti, Kristen Kulp, and Kurt W. Haack 13. Analysis of DNA Strand Cleavage at Abasic Sites Walter A. Deutsch and Vijay Hegde Part VI. Detection of Chromosomal and Genome-Wide Damage 14. Premature Chromosome Condensation in Human Resting Peripheral Blood Lymphocytes without Mitogen Stimulation for Chromosome Aberration Analysis Using Specific Whole Chromosome DNA Hybridization Probes Rupak Pathak and Pataje G. S. Prasanna 15. Mutagen Sensitivity as Measured by Induced Chromatid Breakage as a Marker of Cancer Risk Xifeng Wu, Yun-Ling Zheng, and T. C. Hsu 16. Pulsed-Field Gel Electrophoresis Analysis of Multicellular DNA Double-Strand Break Damage and Repair Nina Joshi and Stephen G. Grant Part VII. Detection and Characterization of Surrogate Gene Mutation 17. Detection of Pig-a Mutant Erythrocytes in the Peripheral Blood of Rats and Mice Vasily N. Dobrovolsky, Xuefei Cao, Javed A. Bhalli, and Robert H. Heflich 18. The Blood-Vased Glycophorin A Human In vivo Somatic Mutation Assay Nicole T. Myers and Stephen G. Grant 19. Flow Cytometric Quantification of Mutant T Cells with Altered Expression of the T-Cell Receptor: Detecting Somatic Mutants in Humans and Mice Seishi Kyoizumi, Yoichiro Kasunoki, and Tomonori Hayashi 20. Analysis of in Vivo Mutation at the HPRT and TK Genes of Mouse Lymphocytes Vasily N. Dobrovolsky, Joseph G. Shaddock, and Robert H. Heflich 21. Quantifying In Vivo Somatic Mutations Using Transgenic Mouse Model Systems Roy R. Swiger 22. The Human T-Cell Cloning Assay: Ide From the Back Cover Molecular Toxicology Protocols, Second Edition aims to bring together a series of articles describing validated methods to elucidate specific molecular aspects of toxicology, the emphasis being on the application of molecular methods to genetic toxicology. The volume is divided into ten parts, roughly corresponding to the spectrum of biomarkers intermediate between exposure and disease outcomes as proposed in molecular epidemiology models. Subjects of these new chapters range from preparation of fluid specimens for analysis of cellular inflammatory responses to genoto