Humana Therapeutic Oligonucleotides: Methods and Protocols: 764 (Methods in Molecular Biology, 764)

This volume in the Methods in Molecular Biology™ series presents established and emerging methods for the application of oligonucleotides as therapeutics. Includes protocols for optimizing cell uptake, assaying gene knockdown, target site selection and more. Review From the reviews:“The book is written for both professionals and novices interested in the field of oligonucleotide therapeutics. It also would be appropriate for graduate students and research scientists interested in this area. … This is an extremely useful resource that provides different ways that oligonucleotides may be applied as therapeutics. Very few books cover the field as thoroughly and as clearly as this one.” (Omer Iqbal, Doody’s Book Reviews, November, 2011) From the Back Cover The field of oligonucleotide therapeutics research is ripe with the prospect of new discoveries. In Therapeutic Oligonucleotides: Methods and Protocols, a selection of established and emerging methods for the application of oligonucleotides as therapeutics are presented, all providing the tools needed to inspire great changes in the field. Divided into twenty-one chapters, this detailed volume meticulously describes vital protocols for optimizing and improving cell uptake, such as photochemical internalization, modified cell penetrating peptides, antibody conjugates, and nanoparticles. Other chapters address quantitation of RNA therapeutics in cells, assaying gene knockdown, selecting the best target site and synthesis of various modified oligonucleotides. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, Therapeutic Oligonucleotides: Methods and Protocols serves as a timely resource for both professionals and novices pursuing research in this exciting and pioneering field.