Humana Kinase Inhibitors: Methods and Protocols: 795 (Methods in Molecular Biology, 795)

In this Methods in Molecular Biology™ volume, experts in kinase biology, drug discovery and clinical research present exemplary methods for addressing the challenges involved in discovery and development of kinase inhibitors both for research and clinical use. Review From the reviews:“This well-organized book is highly recommended to those who are interested in kinase inhibitors in both basic research and clinical practice. It is intended to provide an easily accessible collection of methods and strategies in the discovery and development of kinase inhibitors for research and clinical applications. … chapters include a background and introduction, a list of materials required, and a detailed step-by-step description of the methods including highlights of key steps in the procedure.” (Thomas L. Pazdernik, Doody’s Book Reviews, March, 2012) From the Back Cover Protein and lipid kinases are often the master regulators of cell signaling in eukaryotic systems. The human genome codes for more than 500 of these enzymes and their misregulation has been shown to be involved in the onset and progression of many diseases including cancer and inflammation. Therefore, small molecule kinase inhibitors have become important research tools for the elucidation of the many biological roles of kinases and their mechanisms of action. Kinase inhibitors thus also contribute significantly to the drug pipelines of the pharmaceutical and biotechnology industries and to the growing need to treat cancer and inflammation. In Kinase Inhibitors: Methods and Protocols, experts in kinase biology, drug discovery, and clinical research present a series of exemplary methods that can be used to address the many challenges facing scientists in the discovery and development of kinase inhibitors both for research and clinical use. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and accessible, Kinase Inhibitors: Methods and Protocols aims to provide scientists with modern and relevant methods to accelerate or strengthen their research and drug discovery programs through the utilization of these vital regulators.