Cold Spring Harbor Laboratory Press Transcriptional Regulation in Eukaryotes: Concepts, Strategies, and Techniqes: Concepts, Strategies and Techniques (Manual)

This second edition of the highly successful Transcriptional Regulation in Eukaryotes has been extensively rewritten and updated to discuss new advances in the field and their impact on gene regulation mechanisms. It retains the approach of the first in covering both the conceptual and practical aspects of how to study the regulation of a newly isolated gene and the biochemistry of a new transcription factor. Transcriptional Regulation in Eukaryotes is a powerful textbook and manual for advanced instruction in molecular biology which supplements clearly written text with extensive illustrations puts methods in the context of underlying theory gives expert recommendations on experimental strategies encourages creativity in investigative design explains protocols for essential techniques step by step, with extensive advice on troubleshooting provides the latest methods in use in the field This important and unique book is essential reading for anyone pursuing the analysis of gene expression in model systems or disease states, providing underlying theory and perspective to the newcomer and the latest techniques to the expert. Contents: Preface Overview Abbreviations and Acronyms 1. Transcriptional Regulation and Chromatin Structure and Function in Eukaryotic Cells 2. Initial Strategic Issues 3. Transcription Initiation Site Mapping 4. Functional Assays for Promoter Analysis 5. Identification and Analysis of Distant Control Regions 6. Identifying cis-Acting DNA Elements within a Control Region 7. Identification of DNA-Binding Proteins and Their Genes 8. Confirming the Functional Importance of a Protein DNA Interaction 9. Analysis of Transcription Factor Chromatin Interactions on Endogenous Control Region 10. Identifying and Characterizing Domains of Transcription Regulatory Factors 11. DNA Binding by Regulatory Transcription Factors 12. Transcription and Preinitiation Complex Assembly In Vitro 13. Studying Chromatin Dynamics In Vitro: Chromatin Assembly, Remodeling, and Transcription Index Appendix: Cautions