Academic Press Protocols and Applications in Enzymology

Protocols and Applications in Enzymology provides instruction on the experimental procedures of enzyme isolation techniques, innovative screening techniques, and instrument enabled enzyme assays and their underlying principles, among other protocols. The book serves as a one-stop solution for those working with different enzyme protocols in the fields of biochemistry, microbiology, biotechnology and allied subjects. Each chapter offers a full overview of protocol key resources, materials required, quantifiable and statistical analysis, optimization and troubleshooting, safety considerations, and standards. Applications are discussed across distribution and diversity of microbial enzymes, enzyme screening, enzymes in solid state fermentations, enzyme assays, enzyme kinetics, and biotechnological uses. Review Offers thorough instruction on the experimental procedures and applications of enzymology across biochemistry, microbiology, biotechnology, and allied fields From the Back Cover <p><i>Protocols and Applications in Enzymology </i>describes and provides instruction in the experimental procedures of enzyme isolation techniques, innovative screening techniques, and instrument enabled enzyme assays and their underlying principles, among other protocols. It serves as a one-stop solution for those working with different enzyme protocols in the fields of biochemistry, microbiology, biotechnology, and allied subjects. Each chapter offers a full overview of protocol key resources, materials required, quantifiable and statistical analysis, optimization and troubleshooting, safety considerations, and standards. Applications are discussed across distribution and diversity of microbial enzymes, enzyme screening, enzymes in solid state fermentations, enzyme assays, enzyme kinetics, and a range of other biotechnological uses.</p> About the Author Dr. Seema Anil Belorkar is Assistant Professor at the PG Department of Microbiology and Bioinformatics, Atal Bihari Vajpayee University, Bilaspur, with expertise in bioactive molecule production and enzymology. She has published 20 publications in various national and international journals, and has successfully completed 4 research projects with involvin production of enzymes and their application in agro-industrial wastes. Dr. Belorkar has contributed to 4 chapters in various books published by Springer, Wiley-Blackwell, and Elsevier. She has received 3 awards in the field of teaching and research, and has been a reviewer for articles in BMC Plant Biology, the Indian journal of Pharmaceutical Sciences and other journals of repute. Dr. Sudisha Jogaiah (M.Sc., M.Phil., Ph.D., Post-doc (JSPS, Japan) FNABS) is Assistant Professor and Programme Coordinator at the Laboratory of Plant Healthcare and Diagnostics, PG Department of Biotechnology and Microbiology, Karnatak University, Dharwad, India. The technological quality and expertise of Dr. Jogaiah’s lab is reflected in an extensive track record of publications with over 87 peer-reviewed papers, one national patent and 6 review articles with h-index of 24. He also contributed 18 chapters in various book editions published by Springer, Wiley-Blackwell, and Elsevier. He has expertise in plant defense enzymes and signalling pathways for induction of systemic resistance. He has also edited three books on bioactive molecules involved in plant-microbe interactions and defense. He is the recipient of Fellow of National Academy of Biological Sciences (FNABS) and has received a range of international awards. Dr. Jogaiah has served as editor of Scientific Reports, Plos One, BMC Plant Biology, Frontiers in Plant Science, Annals of Crop Sciences and Agriculture, Journal of Mycology and Plant Pathology, and BioMed Research International.