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Elsevier Proteomics Mass Spectrometry Methods: Sample Preparation, Protein Digestion, and Research Protocols

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Description
Proteomics Mass Spectrometry Methods: Sample Preparation, Protein Digestion, and Research Protocols shares best practices collected across key laboratories and core facilities, taking the reader through key tactics for executing the most usual mass spectrometry experiments. Sections review research making use of MS proteomics experiments, focus on critical sample preparation, cover mammalian cell lines and samples from clinical tissue and biological fluids, discuss subcellular fractionation, provide methods for protein digestion both for in gel and in solution, and delve into key MS proteomics analysis protocols, including label-free LC-MS, TMT and iTRAQ labelled LC-MS, phosphorylation enrichment, ubiquination enrichment, and more. This book is the perfect lab manual for research teams or for use as a new staff training material. Core facility managers may also find it useful for sharing best practices with their staff and researchers. Explores the most common questions new researchers have Guides readers to properly design the workflow for successful integration of mass spectrometry into protein biochemical analyses Provides examples of sample preparation for a number of different materials, mammalian cells, and others

Product Specifications
Brand
Elsevier
Format
paperback
ASIN
0323903959
Category
Books > Subjects > Science, Nature & Maths > Biological Sciences > Biological Science
Domain
Amazon UK
Release Date
13 February 2024
Listed Since
05 January 2023

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No barcode data available

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