£102.69

Humana The CRAC Channel: Methods and Protocols: 1843 Book

Name: Humana The CRAC Channel: Methods and Protocols: 1843 Book
Brand: Humana
Price: 102.53 GBP
Availability: InStock

Biochemistry

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Description

Explore essential cellular and molecular techniques with The CRAC Channel: Methods and Protocols: 1843. Part of the Methods in Molecular Biology series, this volume provides a detailed look at the methodologies used to study and characterize the various components within the CRAC channel signaling pathway. This resource offers practical guidance on diverse laboratory procedures. You will find information on fluorescence-based measurements for tracking CRAC channel activity in cell populations and patch-clamp recording techniques for both STIM-Orai overexpressing cells and native systems. The text also covers protein analysis through western-blotting and co-immunoprecipitation of endogenous STIM/ORAI and their protein partners. Designed for researchers in biochemistry and molecular biology, this book includes specialized topics such as shRNA-mediated gene silencing and the SC-SMD system. It serves as a technical guide for those working to understand the mechanisms of signaling pathways through established scientific protocols.

Key Features

Covers fluorescence-based measurements to study CRAC channel activity within cell populations.

Provides protocols for patch-clamp recording in native systems and STIM-Orai overexpressing cells.

Includes methods for western-blotting and co-immunoprecipitation of endogenous STIM/ORAI protein partners.

Details shRNA-mediated gene silencing techniques for molecular research.

Explains the use of the SC-SMD system for specialized cellular studies.

Part of the successful Methods in Molecular Biology series by Humana.

Product Specifications

Brand: Humana
Format: paperback
ASIN: 1493993704
Category: Books > Subjects > Science, Nature & Maths > Biological Sciences > Biochemistry
Domain: Amazon UK
Release Date: 10 December 2019
Listed Since: 15 August 2019

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Humana The CRAC Channel: Methods and Protocols: 1843 Book

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