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Humana CRAC Channel Methods and Protocols - Volume 1843 (MMB)

Amazon

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Description

This comprehensive volume from the Methods in Molecular Biology series provides detailed laboratory protocols for studying CRAC channel signalling pathways. Researchers will find practical guidance on fluorescence-based measurements of CRAC channel activity in cell populations, patch-clamp recording techniques in both STIM-Orai overexpressing and native systems, western-blotting and co-immunoprecipitation methods for endogenous STIM/ORAI proteins and their binding partners, shRNA-mediated gene silencing approaches, and SC-SMD system applications. Each chapter delivers step-by-step instructions suitable for molecular biology laboratories investigating calcium-release-activated calcium channels and related cellular mechanisms. The established Methods in Molecular Biology format ensures consistency with the series' renowned pedagogical approach, making this volume an essential reference for postgraduate researchers and experienced scientists working in cellular signalling and ion channel biology.

Key Features

Fluorescence-based measurement protocols for CRAC channel activity analysis across cell populations.

Patch-clamp recording techniques for CRAC channels in both STIM-Orai overexpressing and native cellular systems.

Western-blotting and co-immunoprecipitation methods for detecting endogenous STIM/ORAI protein interactions and partners.

shRNA-mediated gene silencing strategies for targeted CRAC channel component suppression.

SC-SMD system applications and protocols integrated throughout the volume.

Methods in Molecular Biology series format providing consistent structure and reproducible laboratory guidance.

Product Specifications

Brand
Humana
Format
paperback
Domain
Amazon UK
Release Date
10 December 2019
Listed Since
15 August 2019

Barcode

No barcode data available

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