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Humana TET Proteins and DNA Demethylation Methods - Humana (2272)

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Description

This comprehensive laboratory manual from Humana explores cutting-edge methods for studying TET proteins and DNA demethylation biology. The volume is organised across five parts, beginning with technologies for detecting and quantifying DNA methylation turnover using massively parallel sequencing, ELISA, and mass spectrometry techniques. Part Two provides data analysis protocols for distinguishing active versus passive DNA demethylation and estimating 5mC and 5hmC levels. Part Three introduces modified CRISPR/Cas9 genome editing systems designed to target DNA demethylation activities. This Methods in Molecular Biology volume (2272) serves as an essential reference for researchers, molecular biologists, and biochemists working with epigenetic modifications, offering practical protocols and contemporary approaches for investigating TET protein function and DNA methylation dynamics in modern molecular biology laboratories.

Key Features

Part One describes detection and quantification technologies for DNA methylation turnover using massively parallel sequencing, ELISA, and mass spectrometry methods.

Part Two provides data analysis protocols for distinguishing active versus passive DNA demethylation and accurate estimation of 5mC and 5hmC levels.

Part Three covers modified CRISPR/Cas9 genome editing systems specifically designed to target DNA demethylation activities in molecular studies.

Humana publication combines laboratory protocols with theoretical background for comprehensive understanding of TET protein biology and function.

Product Specifications

Brand
Humana
Format
hardcover
Domain
Amazon UK
Release Date
20 May 2021
Listed Since
16 October 2020

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