Humana Oligonucleotide Synthesis: Methods and Applications 288

Explore advanced techniques in biotechnology with Oligonucleotide Synthesis: Methods and Applications, volume 288 of the Methods in Molecular Biology series. This Humana publication provides a collection of powerful new methods for oligonucleotide synthesis and the use of modified oligonucleotides. Readers can access detailed protocols, including a novel two-step process for producing high purity, less costly DNA and the synthesis of phosphorothioates using new sulfur transfer agents. The text covers the synthesis of LNA and peptide conjugation methods designed to improve cell-specific targeting and cellular delivery. Additionally, the book examines applications such as triple helix formation, using molecular beacons to monitor PCR amplification, and nuclease footprinting to study sequence-selective binding of small molecules.