Humana Oligonucleotide Synthesis: Methods and Applications 288

Expand your biotechnology toolkit with Oligonucleotide Synthesis: Methods and Applications, part of the Methods in Molecular Biology series. This volume provides a collection of powerful new techniques designed for oligonucleotide synthesis and the practical use of modified oligonucleotides in various biotech fields. Readers can explore advanced protocol highlights, including a novel two-step process for producing high-purity, cost-effective DNA. The text covers the synthesis of phosphorothioates using new sulfur transfer agents and the synthesis of LNA. It also details peptide conjugation methods intended to improve cellular delivery and cell-specific targeting, as well as triple helix formation. Beyond synthesis, the book covers vital applications such as using molecular beacons to monitor PCR amplification processes and utilizing nuclease footprinting to study sequence-selective binding. This resource is an essential reference for professionals working in molecular biology, pharmacy, and biotechnology research.