Humana MicroRNAs and the Immune System - Methods and Protocols

Explore the biological role of microRNAs within the cells of the immune system with this professional scientific resource. MicroRNAs and the Immune System: Methods and Protocols provides expert researchers with access to the latest techniques for studying miRNA expression. This volume covers essential methodologies including splinted ligation, qRT-PCR assays, and high-throughput profiling through cloning, deep sequencing, and microarrays. Detailed chapters outline specific methods to study miRNA functions across various biological scales, ranging from single cell types to entire model organisms. This text serves as a comprehensive guide for understanding miRNA studies in the context of immune system biology and cellular research.