Humana MicroRNAs and the Immune System: Methods and Protocols

Explore the essential role of microRNAs within immune system cell biology. This volume from the Methods in Molecular Biology series provides expert researchers with a comprehensive guide to the latest techniques for studying miRNA expression. Readers can access up-to-date data on essential assays, including splinted ligation and qRT-PCR. The book covers high-throughput profiling methods such as cloning, deep sequencing, and microarrays to support advanced research needs. Detailed chapters outline specific methods to study miRNA functions across various biological scales. These protocols range from analyzing single cell types to investigating entire model organisms. This resource is designed to support scientific study into how miRNAs operate within the immune system and related biological processes.