£130.00

Humana Long Non-Coding RNAs in Cancer (Methods in Molecular Biology)

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Description

This specialized volume provides the technical foundation required to study long non-coding RNAs (lncRNAs) in cancer research. It covers the entire workflow, starting from the initial identification of lncRNA expression signatures to full functional characterization. Researchers can find detailed guidance on using RNAseq, single Cell RNAseq, Phospho RNAseq, and Nanopore Sequencing to identify signatures in cancer tissue or liquid biopsies. The text also explains how to validate these signatures using Real time PCR, digital PCR, or in situ hybridization. For those focused on functional analysis, the book details methods for lncRNA silencing or overexpression using siRNA or CRISPR-based approaches. Additionally, it addresses the use of lipid based nanoparticles for delivery of siRNA. This resource serves as a practical guide for professionals working in molecular biology and oncology research.

Key Features

Covers identification of lncRNA expression signatures in cancer tissue and liquid biopsies using RNAseq and Nanopore Sequencing.

Provides methods for validating lncRNA signatures through Real time PCR, digital PCR, and in situ hybridization techniques.

Includes functional analysis protocols for lncRNA silencing or overexpression using siRNA and CRISPR-based methods.

Explains advanced sequencing technologies including single Cell RNAseq and Phospho RNAseq for cancer research.

Discusses the application of lipid based nanoparticles for the delivery of siRNA in molecular studies.

Product Specifications

Brand
Humana
Format
Hardcover
Domain
Amazon UK
Release Date
24 June 2021
Listed Since
25 February 2021

Barcode

No barcode data available

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