Humana Heterologous Expression of Membrane Proteins 2507

Master the production of membrane proteins for structural studies with this comprehensive technical volume. Published by Humana as part of the Methods in Molecular Biology series, this book provides a detailed roadmap for researchers working with a wide variety of heterologous organisms. Starting with foundational techniques using E. coli for overproduction and purification, the text expands into advanced methodologies. You will find specific protocols for membrane protein production in yeast, insect cells, and mammalian cells. The volume also explores specialized approaches using virus-like particles and acellular systems to meet diverse research needs. Beyond host selection, this guide addresses the technical challenges of protein stability and purification. It describes new detergents and effective alternatives to detergents that facilitate successful membrane protein purification for structural analyses. This resource serves as a practical manual for scientists seeking to optimize their workflows in biochemistry and molecular biology.