£118.31

Humana Heterologous Expression of Membrane Proteins: Methods and Protocols

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Description

This comprehensive Methods in Molecular Biology volume (2507) provides detailed protocols for producing membrane proteins across multiple heterologous expression systems suited for structural studies. The book covers membrane protein overproduction and purification in E. coli, followed by chapters on mammalian expression in yeast, insect cells, and mammalian cells, as well as virus-like particles and acellular systems. It includes guidance on modern detergents and detergent-free alternatives for membrane protein purification and structural analysis, making it an essential reference for biochemists and molecular biologists working with challenging membrane proteins in research and biotechnology applications.

Key Features

Methods in Molecular Biology volume 2507 with protocols for heterologous expression of membrane proteins in E. coli, yeast, insect, and mammalian cell systems.

Covers membrane protein overproduction and purification techniques optimised for structural biology studies and research applications.

Includes chapters on detergent-based and detergent-free approaches for membrane protein solubilisation and purification.

Features protocols for virus-like particle and acellular expression systems as alternative hosts for membrane protein production.

Provides comprehensive coverage of mammalian membrane protein production methods across multiple expression platforms.

Published by Humana as part of the established Methods in Molecular Biology series for laboratory and academic use.

Product Specifications

Brand
Humana
Format
paperback
Domain
Amazon UK
Release Date
02 July 2023
Listed Since
04 June 2023

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