Humana Exon Skipping: Methods and Protocols 867 Book

Exon Skipping: Methods and Protocols (Methods in Molecular Biology, 867) offers a detailed guide for scientists working with advanced genetic analysis. As next generation sequencing techniques become more common, they allow for deeper analysis of exons and introns across multiple genes simultaneously. This capability helps identify mutations that can lead to exon skipping. This volume provides the necessary guidance to move from discovery to functional testing. It covers protocols for testing mutations with a limited set of methods, as well as the specific tools and target genes required for the intentional induction of exon skipping. The text addresses the full translational path, moving from in vitro splicing studies to in vivo tests in animal models. By providing an extensive set of protocols, this book serves as a comprehensive resource for researchers navigating the complexities of molecular biology and gene regulation.