Humana Engineering Natural Product Biosynthesis: Methods and Protocols

Engineering Natural Product Biosynthesis: Methods and Protocols (volume 2489 in the Methods in Molecular Biology series) provides comprehensive guidance on natural products and molecular techniques for their identification and design. This authoritative volume covers heterologous expression techniques, gene disruption strategies, modified pathway regulators, and in-vitro studies essential for modern biosynthesis research. Each chapter follows the proven Methods in Molecular Biology format, offering detailed introductions, complete materials and reagents lists, troubleshooting advice, and step-by-step reproducible protocols. Written by experts in the field, this book equips researchers and practitioners with cutting-edge methods to engineer and optimise natural product biosynthesis pathways, making it an indispensable resource for molecular biologists, biochemists, and pharmaceutical researchers working in natural product development.