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Humana E. coli Gene Expression Protocols - Methods in Molecular Biology

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Description

This comprehensive guide by Peter E. Vaillancourt compiles proven methodologies for leveraging E. coli's rapid and cost-effective protein expression capabilities. The book addresses two primary research focuses: utilising E. coli vectors and strains to produce pure, functional recombinant proteins, and employing E. coli as a host system for functional screening of extensive protein and peptide libraries. Readers gain access to cutting-edge techniques for achieving high-level expression and purification of soluble, functional recombinant proteins alongside essential functional screening methods. Ideal for molecular biologists, research scientists and laboratory professionals working with recombinant protein production and protein engineering, this volume 205 in the Methods in Molecular Biology series delivers practical protocols and detailed methodologies that accelerate research productivity and improve protein yields.

Key Features

E. coli vectors and strains are explored in depth for generating pure, functional recombinant proteins with reproducible results.

The book demonstrates rapid high-level expression techniques that maximise protein production efficiency in E. coli host systems.

Functional screening protocols enable researchers to evaluate large collections of proteins and peptides using E. coli as the expression host.

Purification methodologies for soluble and functional recombinant proteins ensure researchers obtain high-quality proteins suitable for downstream applications.

Volume 205 in the Methods in Molecular Biology series provides peer-reviewed protocols written by experienced molecular biologists and researchers.

Product Specifications

Brand
Humana
Format
paperback
Domain
Amazon UK
Release Date
29 December 2011
Listed Since
01 October 2010

Barcode

No barcode data available

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