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Humana Directed Enzyme Evolution: Screening and Selection Methods

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Description

Directed enzyme evolution is a two-step process involving the generation of molecular diversity and the identification of mutant sequences that meet specific functional criteria. This second step is often the most difficult part of the process. Even with a well-designed starting library, the success of isolating useful clones depends on the effectiveness of your screening strategy. This volume, part of the Methods in Molecular Biology series (Volume 230), focuses on the essential strategies needed to bridge the gap between creating diversity and finding results. It addresses the need for high-throughput screening methods to increase the likelihood of finding useful clones within an ensemble of mutant sequences. For researchers working in biological sciences, understanding these selection methods is necessary to improve the efficiency of protein engineering and molecular evolution projects.

Key Features

Focuses on the critical second step of directed evolution: identifying specific proteins within an ensemble of mutant sequences.

Addresses the challenges of isolation by discussing effective screening strategies for diverse starting libraries.

Provides insight into high-throughput methods designed to increase the probability of finding useful clones.

Part of the recognized Methods in Molecular Biology series, specifically Volume 230.

Designed for professionals and students working in biological sciences and protein engineering.

Product Specifications

Brand
Humana
Format
paperback
Domain
Amazon UK
Release Date
10 November 2010
Listed Since
04 July 2010

Barcode

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