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Humana Directed Enzyme Evolution: Screening & Selection Methods

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Description

Directed Enzyme Evolution: Screening and Selection Methods is a comprehensive guide from Humana's Methods in Molecular Biology series that addresses the critical second step of directed evolution - identifying proteins with desired functions from diverse mutant libraries. This volume explores how to design and implement high-throughput screening strategies that maximise the chances of isolating useful clones from even the most cleverly constructed starting libraries. The book examines both the theoretical foundations and practical applications of screening and selection methods essential for successful protein engineering. Whether you are developing novel enzymes for industrial applications or studying protein function through directed evolution, this Methods in Molecular Biology resource provides the expertise needed to overcome the screening bottleneck that often limits successful clone isolation and characterisation.

Key Features

Directed Enzyme Evolution focuses on screening and selection as the critical limiting step in iterative protein engineering workflows.

Humana's Methods in Molecular Biology series volume 230 combines theoretical principles with practical laboratory protocols for enzyme evolution.

The book emphasises high-throughput screening strategies designed to increase the probability of finding useful variant clones.

Addresses the challenge of isolating functional proteins from large libraries of mutant sequences generated through molecular diversity techniques.

Provides expert guidance on designing effective screens that complement even the most sophisticated molecular library construction methods.

Suitable for researchers in protein engineering, molecular biology, and biotechnology seeking systematic screening approaches.

Product Specifications

Brand
Humana
Format
paperback
Domain
Amazon UK
Release Date
10 November 2010
Listed Since
04 July 2010

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