Humana CRISPR Gene Editing: Methods and Protocols (1961)

Master the complexities of genome engineering with CRISPR Gene Editing: Methods and Protocols. This specialized volume from the Methods in Molecular Biology series provides a practical roadmap for researchers looking to navigate the selection of CRISPR-Cas systems. By following these user-friendly guidelines, you can identify target sites that offer high activity and specificity for your specific research needs. The text offers a comprehensive look at essential laboratory processes, including gRNA design, delivery methods, and precise activity quantification through indel quantification. Beyond foundational techniques, this guide provides practical applications for modern science, covering gene editing in human pluripotent stem cells, primary cells, gene therapy, and genetic screening. Each chapter is structured to support your workflow, providing necessary introductions and detailed lists to ensure you have the right information for every stage of your molecular biology projects.