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Humana CRISPR Gene Editing: Methods and Protocols - Volume 1961

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Description

CRISPR Gene Editing: Methods and Protocols is a comprehensive guide in the Methods in Molecular Biology series that equips researchers with practical strategies for selecting optimal CRISPR-Cas systems and identifying high-activity, high-specificity target sites. This volume covers essential techniques including gRNA design, delivery methods, and activity quantification through indel analysis, whilst providing detailed examples of CRISPR applications in human pluripotent stem cells, primary cell cultures, gene therapy development, and genetic screening workflows. Each chapter offers clear introductions to key topics alongside necessary materials and protocols, making this resource invaluable for molecular biologists, genetic researchers, and scientists implementing CRISPR-based approaches in laboratory and therapeutic settings.

Key Features

CRISPR-Cas system selection strategies help researchers identify the most suitable gene editing approach for their specific research objectives.

gRNA design methods optimise guide RNA targeting to achieve high activity and specificity at intended genomic sites.

CRISPR delivery techniques cover multiple approaches for introducing gene editing components into diverse cell types.

Indel quantification protocols enable precise measurement of CRISPR activity and off-target effects in edited cells.

Applications in human pluripotent stem cells and primary cultures provide reproducible protocols for advanced cell engineering.

Gene therapy and genetic screening examples demonstrate real-world implementation of CRISPR technology in research and clinical contexts.

Product Specifications

Brand
Humana
Format
hardcover
Domain
Amazon UK
Release Date
26 March 2019
Listed Since
01 December 2018

Barcode

No barcode data available

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