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Humana Cell Cycle Checkpoints: Methods and Protocols - Humana 2267

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Description

Cell Cycle Checkpoints: Methods and Protocols is volume 2267 in the Methods in Molecular Biology series, offering comprehensive research techniques for studying cellular checkpoint mechanisms and their connections to human disease. This volume presents step-by-step protocols covering p53 post-translational modifications via western blotting, CHK1 cellular localisation using immunofluorescence microscopy, DNA affinity purification methods, siRNA-mediated gene knockdown in vitro, and calreticulin exposure detection during mitotic catastrophe. Each chapter follows the proven Methods in Molecular Biology format, providing topic introductions, complete materials and reagents lists, and detailed procedural instructions. Whether you work in immunology, cell biology, or disease research, this Humana-published reference equips laboratory professionals with validated experimental approaches for investigating cell cycle control pathways and their biological significance.

Key Features

Covers p53 post-translational modification analysis using western blotting techniques to identify critical regulatory changes.

Includes CHK1 cellular localisation protocols employing immunofluorescence microscopy for subcellular distribution mapping.

Presents DNA affinity purification methods for isolating and studying protein-DNA interactions in cell cycle regulation.

Details siRNA knockdown procedures for targeted in vitro gene silencing and functional analysis in cell culture.

Describes calreticulin exposure detection assays relevant to mitotic catastrophe and cell death mechanisms.

Product Specifications

Brand
Humana
Format
hardcover
Domain
Amazon UK
Publication Date
31 March 2021
Listed Since
03 September 2020

Barcode

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