Elsevier RNA Turnover in Eukaryotes: Nucleases & mRNA Decay - Vol. 448

This Methods in Enzymology volume explores the critical mechanisms of RNA turnover in eukaryotic cells, examining the protein-RNA complexes responsible for essential biological functions including RNA processing, folding, and translation. Messenger RNA (mRNA) decay has emerged as a significant control point in gene expression regulation, and this comprehensive resource details the protein factors, cofactors, and mRNA instability elements that orchestrate decay pathways. The book systematically covers nucleases and analytical techniques used to study mRNA degradation, providing researchers with the foundational knowledge and methodological frameworks needed to understand how cells regulate gene expression through controlled mRNA turnover and identify novel regulatory mechanisms in eukaryotic RNA metabolism.