£130.75

Academic Press Lipids and Membranes Volume 729 - Methods in Enzymology

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Description

Lipids and Membranes: Metabolism, Lipidation, and Lipid-Protein Interactions, Volume 729 from Academic Press delivers comprehensive research methods for studying biological lipids and biomembranes. This volume in the Methods in Enzymology series covers essential techniques including de novo chemoenzymatic construction of lipid membranes, photoaffinity labeling for cholesterol metabolism, expression and characterisation of Phospholipase Cb and Ce enzymes, and assembling Retromer-coated membrane tubules for in vitro structure-function studies. Additional chapters address quantitative analysis of S-acylation, inhibition of palmitoyltransferases, and exploitation of protein lipidation strategies. Designed for biochemists, researchers, and scientists working in membrane biology and lipid metabolism, this resource provides state-of-the-art experimental protocols and methodologies essential for advanced laboratory work.

Key Features

Volume 729 covers de novo chemoenzymatic construction of lipid membranes using modern synthetic approaches for biomembrane research.

Includes photoaffinity labeling techniques specifically designed for studying cholesterol metabolism in cellular systems.

Features detailed protocols for expression and characterisation of Phospholipase Cb and Ce enzymes with functional analysis methods.

Provides methods for assembling Retromer-coated membrane tubules enabling in vitro structure-function studies of membrane dynamics.

Contains quantitative analysis techniques for measuring S-acylation modifications on proteins within lipid environments.

Offers strategies for inhibition of palmitoyltransferases to investigate protein lipidation pathways and cellular processes.

Product Specifications

Format
hardcover
Domain
Amazon UK
Release Date
10 January 2026
Listed Since
06 August 2025

Barcode

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