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Elsevier Lipidomics and Bioactive Lipids: Volume 434 Methods in Enzymology

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Description

This volume in the established Methods in Enzymology series presents comprehensive methods for studying lipids through mass spectrometry techniques. The collection covers essential analytical approaches including Phospholipase A1 assays with radio-labeled substrates, real-time cell assays for Phospholipases A2 using fluorogenic phospholipids, and biochemical analysis of Phospholipase C interactions with G proteins. Readers will find detailed protocols for measuring Phospholipase D activity, Autotaxin and Lysophospholipase D activity, Platelet-Activating Factor responses, and quantitative measurement of PtdIns(3,4,5)P3. This academic resource is designed for researchers and practitioners working in lipidomics, cell signalling, and biochemical analysis who require reliable, standardised methods for lipid research and pharmacological targeting applications.

Key Features

Phospholipase A1 assays utilising radio-labeled substrates and mass spectrometry detection for precise enzyme activity measurement.

Real-time cell assays of Phospholipases A2 employing fluorogenic phospholipids to monitor enzyme function in living cells.

Biochemical analysis protocols for Phospholipase C interactions with G proteins and Phospholipase D activity quantification.

Methods for measuring Autotaxin and Lysophospholipase D activity alongside Platelet-Activating Factor analysis.

Quantitative measurement techniques for PtdIns(3,4,5)P3 and comprehensive lipid signalling pathway characterisation.

Product Specifications

Format
hardcover
Domain
Amazon UK
Release Date
23 November 2007
Listed Since
10 August 2007

Barcode

No barcode data available

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