Elsevier Enzyme Activity in Single Cells - Methods in Enzymology Vol 628

Enzyme Activity in Single Cells, Volume 628, is the latest instalment in the Methods in Enzymology series from Academic Press, offering cutting-edge research into cellular physiology and single-cell analysis techniques. This volume presents comprehensive coverage of spatial and temporal resolution methods for studying caspase waves during apoptosis in Xenopus eggs, metabolic complex organisation within cells, and advanced imaging approaches for measuring cellular efflux and biomolecular delivery. The book includes chapters on slide-based single-cell enzyme assays and integrated continuous-flow microfluidic systems, providing researchers with practical methodologies for investigating enzyme activity at the cellular level. Essential for biochemists, cell biologists and researchers working in molecular and cellular science, this volume combines theoretical foundations with hands-on experimental protocols designed to advance understanding of enzyme function in living cells.