Elsevier Cilia: From Mechanisms to Disease Part B - Volume 176

Cilia: From Mechanisms to Disease Part B is Volume 176 of the Methods in Cell Biology series, offering comprehensive research methods and insights into ciliary biology. This academic text covers essential topics including gene function dissection in model organisms, characterization of genetic mutants, transcriptional adaptation in zebrafish, and the use of human LUHMES and NES cells as neuronal ciliary models. The volume explores fixation methods and immunolabelling techniques for cilia proteins in both ciliary and extraciliary locations, single-molecule imaging approaches within primary cilia, and analytical methods for primary cilia assessment in mouse cardiac lesion models. Researchers and cell biologists will find detailed protocols for studying motile cilia and understanding ciliary mechanisms from molecular and disease perspectives. Published by Academic Press, this reference provides advanced methodologies essential for contemporary ciliary research and cell biology applications.