Elsevier CAR T Cells: Development, Characterization - Volume 167

MCB: CAR T Cells: Development, Characterization and Applications, Volume 167 in the Methods in Cell Biology series, presents cutting-edge research and practical methodologies for engineered CAR T-cell therapy. This comprehensive volume covers high-efficiency genetic modification using CRISPR/Cpf1 systems, biodistribution analysis of chimeric antigen receptor-modified T cells against CD19 in NSG mice, and multiple generation techniques including lentiviral vectors and gamma-retroviral vectors. Featuring chapters on flow cytometry detection and quantification methods, this resource combines theoretical foundations with hands-on laboratory protocols essential for researchers developing next-generation immunotherapies. Ideal for cell biologists, immunologists, and biomedical scientists working in CAR T-cell research and therapeutic applications.