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Elsevier Biological NMR Part B - Volume 615 Methods in Enzymology

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Description

Biological NMR, Part B represents the latest volume in the prestigious Methods in Enzymology series from Academic Press, delivering cutting-edge research and practical techniques for studying biomolecular structure and function. This comprehensive volume covers essential NMR methodologies including protein methyl and aromatic labeling strategies, membrane protein expression in yeast systems, metal contamination management with His-tag proteins, and advanced host systems such as bicelles, nanodiscs and micelles. Researchers will find detailed chapters addressing post-translational modifications including phosphorylation and lipidation, innovative screening platforms for receptor-ligand discovery, and solution spectroscopy applications. The book explores large protein strategies, nuclear Overhauser effect dynamic polarisation (ONDP) hydration studies, fluorine-19 NMR techniques with hydration analysis, and reverse micelle approaches for hydration characterisation. Non-uniform sampling (NUS) data collection and analysis methods are thoroughly presented to optimise experimental workflows. This volume serves as an essential reference for biochemists, structural biologists and researchers applying NMR spectroscopy to biological systems.

Key Features

Biological NMR Part B covers protein methyl labeling and aromatic labeling techniques for enhanced NMR spectroscopy applications.

Membrane protein expression methods using yeast host systems are detailed throughout the volume for practical implementation.

Advanced host technologies including bicelles, nanodiscs and micelles are comprehensively explained for diverse sample preparation needs.

Post-translational modification chapters address phosphorylation and lipidation strategies critical for biological NMR studies.

Screening platform information for receptor-ligand discovery enables high-throughput NMR-based applications in drug research.

Large protein strategies and non-uniform sampling (NUS) data collection methods optimise analysis of complex biomolecules.

Hydration characterisation techniques spanning fluorine-19 NMR, ODNP and reverse micelle approaches provide comprehensive coverage.

Product Specifications

Format
hardcover
Domain
Amazon UK
Release Date
23 January 2019
Listed Since
13 June 2018

Barcode

No barcode data available

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