Introduction to Laboratory Methods in Molecular, Cell, and Developmental Biology (Experimental Biology)

~ Laboratory Methods in Cell Biology ~ This book describes some common methods in the cell biology laboratory. The methods are modular and can be combined to make more complex laboratory protocols. Table of Contents Dissociating Tissue Single Cell Suspension Organelle Isolation Homogenization Sectioning Tissue Living :: Vibratome Frozen :: Cryostat Paraffin Wax :: Microtome Purification of Molecular Classes DNA :: Phenol/Chloroform Extraction RNA :: Trizol Extraction Protein :: TCA Precipitation Non-Polar Metabolites :: Folch Extraction Polar Metabolites:: Folch Extraction Purification of Specific Molecules DNA :: Affinity Purification RNA:: Affinity Purification Protein :: Immunoprecipitation Non-Polar Metabolites :: Chromatography Polar Metabolites:: Chromatography Purification via Binding Partner DNA → DNA DNA → Protein RNA → RNA RNA → Protein Protein → DNA :: Chromatin Immunoprecipitation Protein → RNA Protein → Protein :: Co-Immunoprecipitation Synthesis Rate of Molecular Classes DNA RNA Protein Non-Polar Metabolites Polar Metabolites Synthesis Rate of Specific Molecules DNA RNA Protein Non-Polar Metabolites Polar Metabolites Amount of Molecular Classes DNA :: Nanodrop RNA:: Nanodrop Protein :: Bicinchoninic Acid Non-Polar Metabolites Polar Metabolites Amount of Specific Molecules DNA :: PCR RNA:: PCR Protein :: Western Blot Non-Polar Metabolites :: GC-MS Polar Metabolites:: GC-MS Enzymes Degradation Rate of Molecular Classes DNA RNA Protein Non-Polar Metabolites Polar Metabolites Degradation Rate of Specific Molecules DNA RNA Protein Non-Polar Metabolites Polar Metabolites Location of Molecular Classes DNA RNA Protein Non-Polar Metabolites Polar Metabolites Location of Specific Molecules DNA :: In-Situ Hybridization RNA:: In-Situ Hybridization Protein :: Immunohistochemistry Non-Polar Metabolites Polar Metabolites [ 106 pages ]