3B Scientific Experiment kit: Electrophoresis and DNA Fragment Length Determination Lab Activity

Restriction enzymes are enzymes that are capable to cleaving or cutting DNA molecules in very specific spots based on the DNA molecule’s sequence of bases

Students will perform agarose electrophoresis on three DNA samples that have already been treated with restriction enzymes

Students will learn about the process of DNA electrophoresis as well as about the techniques associated with the process such as setting up an agarose gel loading DNA samples in the agarose gel and staining the gel in order to visualize the DNA bands

There is enough DNA to run 10 gels

Needed but not supplied are agarose electrophoresis chambers power supplies and micropipettes capable of measuring 10µL