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ÉDITIONS UNIVERSITAIRES EUROPÉENNES Rôle du TLR2 dans l’hépatite fulminante induite par le virus MHV-3

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Description

Afin de déterminer le rôle du TLR-2 dans l’aggravation de l’hépatite aigüe virale du modèle MHV3, des infections avec le virus MHV3 ont été réalisées in vivo chez des souris C57BL/6 et des souris invalidées pour le gène tlr2 et in vitro dans des macrophages et des hépatocyte infectés avec le virus MHV3 et le virus moins virulent MHV-A59. Les niveaux de transcription et de traduction des senseurs microbiens, des IFN-I, des cytokines et/ou des chimiokines ont été évalués. Des traitements aux shRNA pour le TLR2 et/ou le CEACAM1a ou d’inhibiteurs des voies d’endocytose avant infection ont également été étudiés. Les résultats révèlent le rôle stimulateur du TLR2 de la réplication virale et des niveaux de cytokines pro-inflammatoires IL-6 et TNF-α et des chimiokines CXCL1, CXCL10 et CCL2. Un nouveau mécanisme d’échappement du MHV3 aux senseurs viraux dépendant du TLR2 a également été mis en évidence dans les macrophages et les hépatocytes. Ces différents travaux révèlent un nouveau rôle du TLR2 lors d’infections virales en aggravant la réponse inflammatoire tout en protégeant le virus des autres senseurs de la réponse immune innée.

Product Specifications

Format
paperback
Domain
Amazon UK
Release Date
22 July 2015
Listed Since
14 August 2015

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